Journal:

Article Title: Evolution of the Major Pilus Gene Cluster of Haemophilus influenzae

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Growth of E. coli and H. influenzae and purification of E. coli plasmids were as previously described ( 25 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Source a Relevant properties Reference(s) E. coli DH5α GIBCO BRL Host for E. coli plasmids E. coli plasmids Bluescript pSK − Stratagene pHic Cloned 468-bp fragment of R3001 purE-pepN region, obtained by PCR amplification with primers purE-F and pepN-R followed by subcloning of Alu I partial digestion fragment into Sma I site of pSK− This study pMCC1 Pst I (5.2-kb) fragment of the Hib Eagan hif operon in pUC19, subcloned from a cosmid library; includes the purE-hifA junction, hifA , hifB , and the 5′ end of hifC This study; 13 H. influenzae strains Eagan (E1A) CSF from meningitis patient Type b, streptomycin resistant, virulent in infant rat model 38 Rd (R906) = Goodgal Nonencapsulated derivative of type d strain; avirulent in rat model 8 INT1 Blood from meningitis patient Nonencapsulated; biotype V; virulent in rat model 31 R539 (ATCC 9006) ATCC Reference strain of serotype a R538 (ATCC 9795) ATCC Reference strain of serotype b R540 (ATCC 9007) ATCC Reference strain of serotype c R541 (ATCC 9008) ATCC Reference strain of serotype d R542 (ATCC 8142) ATCC Reference strain of serotype e R543 (ATCC 9796) ATCC Reference strain of serotype f U11 CSF Streptomycin-resistant derivative of U1 (Ramirez); nonencapsulated; avirulent in infant rat assay 38 R1965 (NCTC 8143) NCTC HK389; type strain of H. influenzae ; nonencapsulated, biotype II 22 R1967 (ATCC 11116) ATCC Type strain of H. influenzae biotype aegyptius R2140 Blood H. influenzae biotype aegyptius isolate from BPF; hemagglutinating (CDC F3031) 6 R2141 Blood H. influenzae biotype aegyptius isolate from BPF; nonhemagglutinating (CDC F3035) 6 R2777 CSF, from invasive disease Nonencapsulated This study R3001 Bronchial lavage of cystic fibrosis patient Nonencapsulated 25 C2836 Otitis media Nonencapsulated This study C2840 Tracheal infection in newborn Nonencapsulated This study C2843 Otitis media Type b This study C2853 Sputum from cystic fibrosis patient Nonencapsulated 25 C2859 CSF Nonencapsulated 25 C2861 CSF Nonencapsulated 25 Open in a separate window a ATCC, American Type Culture Collection; NCTC, National Collection of Type Cultures.

Techniques: Plasmid Preparation, Clone Assay, Amplification, Subcloning, Infection